2NZ2
Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95373 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 95.940, 117.470, 155.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.410 - 2.400 |
| R-factor | 0.19757 |
| Rwork | 0.194 |
| R-free | 0.26475 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vl2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.858 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.152 | 0.268 |
| Number of reflections | 17358 | |
| <I/σ(I)> | 3.98 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 16% PEG 3350, 0.15M DL-MALIC ACID, pH 7.0, VAPOR DIFFUSION, temperature 293K |
