2NZ0
Crystal structure of potassium channel Kv4.3 in complex with its regulatory subunit KChIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-11-09 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 112.186, 112.213, 141.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.200 |
| R-factor | 0.26458 |
| Rwork | 0.261 |
| R-free | 0.31015 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.879 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.062 | 0.146 |
| Number of reflections | 14680 | |
| <I/σ(I)> | 10.8 | 1.8 |
| Completeness [%] | 97.4 | 98 |
| Redundancy | 5.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 296 | 2.0 M NaH2PO4/K2HPO4, 0.2 M Li2SO4 and 0.1 M CAPS, pH 10.5, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
