2NXQ

Crystal structure of calcium binding protein 1 from Entamoeba histolytica: a novel arrangement of EF hand motifs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-23
Detector	ADSC QUANTUM 4
Spacegroup name	P 63
Unit cell lengths	95.250, 95.250, 64.996
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.400
Rwork	0.258
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB code 1CLL: n-terminal domain
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.450
High resolution limit [Å]	2.370	5.100	2.370
Rmerge	0.071	0.047	0.386
Number of reflections	12484
<I/σ(I)>	15.5
Completeness [%]	91.2	90	73.9
Redundancy	4.5	3.6	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	289	60% MPD, 5mM CaCl2, 50mM sodium acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K