2NXQ
Crystal structure of calcium binding protein 1 from Entamoeba histolytica: a novel arrangement of EF hand motifs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-23 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.250, 95.250, 64.996 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| Rwork | 0.258 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1CLL: n-terminal domain |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.450 |
| High resolution limit [Å] | 2.370 | 5.100 | 2.370 |
| Rmerge | 0.071 | 0.047 | 0.386 |
| Number of reflections | 12484 | ||
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 91.2 | 90 | 73.9 |
| Redundancy | 4.5 | 3.6 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 289 | 60% MPD, 5mM CaCl2, 50mM sodium acetate, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
