2NXP
Structure of NTD2 domain of the human TAF5 subunit of TFIID
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Detector technology | CCD |
Collection date | 2005-10-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9797, 1.0199 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.620, 61.829, 133.357 |
Unit cell angles | 90.00, 105.16, 90.00 |
Refinement procedure
Resolution | 64.420 - 2.170 |
R-factor | 0.21507 |
Rwork | 0.213 |
R-free | 0.26252 |
Structure solution method | MAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.722 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.400 | |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.102 | 0.065 |
Number of reflections | 76736 | |
<I/σ(I)> | 9.3 | 2.5 |
Completeness [%] | 97.8 | 97.9 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.1 | 295 | 0.1M Tris-HCl, 0.6M calcium chloride, 20% PEG 4000, pH 7.1, VAPOR DIFFUSION, temperature 295K |