2NXE
T. thermophilus ribosomal protein L11 methyltransferase (PrmA) in complex with S-Adenosyl-L-Methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | H 3 |
| Unit cell lengths | 187.179, 187.179, 45.189 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.20265 |
| Rwork | 0.201 |
| R-free | 0.22396 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nxc |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.705 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | COMO |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.046 | 0.434 |
| Number of reflections | 59391 | |
| <I/σ(I)> | 19.13 | 2.1 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | 190 mM Calcium chloride dihydrate, 95mM HEPES-Na, pH 7.5, 26.6% v/v PEG400, 5% glycerol, microbatch under oil, temperature 277K |
