2NWJ
Structure of the complex of C-terminal lobe of bovine lactoferrin with disaccharide at 1.75 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-06 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.960, 49.840, 64.810 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 19.820 - 2.250 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b17 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 17703 | |
| Completeness [%] | 99.4 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | HEPES, PEG 550 Monomethyl ether, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
