2NWH
Carbohydrate kinase from Agrobacterium tumefaciens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-13 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.930, 75.930, 103.608 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.700 - 1.860 |
| R-factor | 0.1966 |
| Rwork | 0.194 |
| R-free | 0.23310 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.548 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.906 | |
| Number of reflections | 29531 | |
| <I/σ(I)> | 41.3 | 2.04 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 16.2 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M calcium chloride, 7% PEG 3350, 4% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
