2NWD

Structure of chemically synthesized human lysozyme at 1 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	56.261, 61.133, 32.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.040
R-factor	0.13361
Rwork	0.133
R-free	0.15222
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jsf see ref. K. Harada Y. abe M. Muraki Proteins 1998 30 232.
RMSD bond length	0.017
RMSD bond angle	1.716
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CCP4
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.040
Number of reflections	53675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.9	293	c 6 mg/mL, 60 mM LiCl, 1.25 mM Hepes, 15 mM sodium phosphate, 1.25 M NaCl , pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K