2NVO
Crystal structure of Deinococcus radiodurans RO (RSR) protein
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Detector technology | CCD |
| Collection date | 2005-08-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97934, 0.97926, 0.97939, 0.94927 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.556, 87.610, 70.623 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.890 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26200 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.062 | 0.685 |
| Number of reflections | 48610 | |
| <I/σ(I)> | 18.9 | 1.64 |
| Completeness [%] | 98.0 | 85.3 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.1Mimidazole, 12% PEG 8000,0.2M calcium acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
