2NVK
Crystal Structure of Thioredoxin Reductase from Drosophila melanogaster
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 104 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-18 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 151.487, 151.487, 134.259 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.203 |
| Rwork | 0.198 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h6v |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.359 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 22987 | |
| <I/σ(I)> | 13.2 | 2.78 |
| Completeness [%] | 98.7 | 90.3 |
| Redundancy | 7.85 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 200 mM Succinate, 22% PEG 6000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
