2NTX
Prone8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 114.339, 114.339, 318.964 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.740 - 2.200 |
| R-factor | 0.24496 |
| Rwork | 0.244 |
| R-free | 0.27030 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.580 |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.300 | |
| High resolution limit [Å] | 2.200 | 15.000 | 2.200 |
| Rmerge | 0.085 | 0.037 | 0.452 |
| Number of reflections | 62938 | ||
| <I/σ(I)> | 20.98 | 47.1 | 4.2 |
| Completeness [%] | 99.2 | 92 | 95.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 740mM NaCl, 100mM sodium citrate, pH 5.6, vapor diffusion, hanging drop, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 740mM NaCl, 100mM sodium citrate, pH 5.6, vapor diffusion, hanging drop, temperature 293K |
