2NTE
Crystal Structure of the BARD1 BRCT Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-10 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9784 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.441, 75.797, 116.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.870 - 1.900 |
| R-factor | 0.19871 |
| Rwork | 0.196 |
| R-free | 0.24743 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 40185 | |
| <I/σ(I)> | 30.7 | 4.3 |
| Completeness [%] | 98.3 | 91.7 |
| Redundancy | 7.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM MES, 20% PEG 8K, 150mM Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
