2NTD
Human fibroblast growth factor-1 (140 amino acid form) with Cys117Val/Pro134Cys mutations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 104 |
| Detector technology | CCD |
| Collection date | 2005-07-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.600, 108.330, 60.920 |
| Unit cell angles | 90.00, 104.97, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.520 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jy0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.140 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.101 | 0.351 |
| Number of reflections | 25003 | |
| <I/σ(I)> | 13.7 | 2.96 |
| Completeness [%] | 98.0 | 95.3 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.6M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
