2NSN
Crystal structure of Caspace Activation and Recruitment Domain (CARD) of NOD1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 170 |
| Detector technology | CCD |
| Collection date | 2006-07-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 40.141, 40.141, 150.748 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.780 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dgn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.340 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.6L) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.380 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.355 |
| Total number of observations | 11350 | |
| Number of reflections | 8946 | |
| <I/σ(I)> | 21.3 | 4.8 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 13.26 | 13.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.7 | 277 | 15% PEG 3000, 100mM acetate, 20mgs/ml protein, pH 4.7, VAPOR DIFFUSION, temperature 277K |
