2NS9
Crystal structure of protein APE2225 from Aeropyrum pernix K1, Pfam COXG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.730, 33.082, 78.442 |
| Unit cell angles | 90.00, 121.40, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.21974 |
| Rwork | 0.217 |
| R-free | 0.27752 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.916 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.930 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.077 | 0.327 |
| Number of reflections | 32448 | |
| <I/σ(I)> | 10.7 | 2.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 6.9 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22% PEG 3350, 0.2M Ammonium Acetate, 0.1M Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
