2NS8
How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-13 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.890, 200.590, 65.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.550 |
| R-factor | 0.211 |
| Rwork | 0.206 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tct |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.710 | |
| High resolution limit [Å] | 2.550 | 5.370 | 2.550 |
| Rmerge | 0.090 | 0.047 | 0.483 |
| Number of reflections | 33932 | ||
| <I/σ(I)> | 14.93 | 27.1 | 3.7 |
| Completeness [%] | 99.4 | 99.8 | 98.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 292 | 4M sodium formate, 0.1M Tris-HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
