2NS6

Crystal Structure of the Minimal Relaxase Domain of MobA from Plasmid R1162

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CAMD BEAMLINE GCPCC
Synchrotron site	CAMD
Beamline	GCPCC
Temperature [K]	100
Detector technology	CCD
Collection date	2005-04-22
Detector	MAR CCD 165 mm
Spacegroup name	P 41 21 2
Unit cell lengths	47.967, 47.967, 165.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.238
R-free	0.28700
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.205
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.046	0.170
Number of reflections	11903
<I/σ(I)>	17.1	10.5
Completeness [%]	97.8	92.9
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	277	21-27% PEG4000, 0.1 M CAPS, 0.2 M NaCl, 10 mM MnCl2, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	10	277	21-27% PEG4000, 0.1 M CAPS, 0.2 M NaCl, 10 mM MnCl2, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K