2NS0
Crystal structure of protein RHA04536 from Rhodococcus sp
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-21 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97907, 0.97921 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.683, 53.165, 90.062 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.040 - 2.005 |
| R-factor | 0.19016 |
| Rwork | 0.189 |
| R-free | 0.21590 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.682 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.110 |
| Number of reflections | 6142 | |
| <I/σ(I)> | 33.36 | 11.5 |
| Completeness [%] | 96.8 | 77 |
| Redundancy | 6.5 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG3350 25% , Bis-Tris 0.1M pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
