2NRX
Crystal structure of the C-terminal half of UvrC, in the presence of sulfate molecules
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-20 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.359, 81.015, 99.619 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nrr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.606 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 0.337 |
| Number of reflections | 42886 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 97.2 | 94.8 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 1.8 M ammonium sulfate, 0.05 M Tris (pH 8.5), 0.025 M magnesium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
