2NRQ
Crystal structure of protein SSO0741 from Sulfolobus solfataricus, Pfam DUF54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 127.540, 127.540, 61.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.430 - 2.600 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.30300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.055 | 0.354 |
| Number of reflections | 6695 | |
| <I/σ(I)> | 14.1 | 3.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.2 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | Sodium Formate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
