2NRN
Self-assembly of coiled-coil tetramers in the 1.40 A structure of a leucine-zipper mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-24 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 20.117, 87.875, 35.509 |
| Unit cell angles | 90.00, 103.83, 90.00 |
Refinement procedure
| Resolution | 43.940 - 1.400 |
| R-factor | 0.18506 |
| Rwork | 0.183 |
| R-free | 0.21912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ipz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.148 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.940 | 1.430 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.057 | 0.451 |
| Number of reflections | 22711 | |
| <I/σ(I)> | 14.1 | 3 |
| Completeness [%] | 95.8 | 91.5 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | 0.1M sodium citrate, 1M ammonium dihydrophosphate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K |
