2NRL
Blackfin tuna myoglobin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.985, 51.976, 38.781 |
| Unit cell angles | 90.00, 103.96, 90.00 |
Refinement procedure
| Resolution | 30.000 - 0.910 |
| R-factor | 0.1487 |
| Rwork | 0.149 |
| R-free | 0.14870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1myt |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.445 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 0.940 |
| High resolution limit [Å] | 0.910 | 0.910 |
| Number of reflections | 92131 | |
| <I/σ(I)> | 16 | 3.1 |
| Completeness [%] | 98.2 | 82.5 |
| Redundancy | 7.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.2 M potassium/sodium tartrate, 0.1 M sodium citrate, and 2.0 M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
