2NRF
Crystal Structure of GlpG, a Rhomboid family intramembrane protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-09-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 31 |
| Unit cell lengths | 59.458, 59.458, 118.048 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.600 |
| R-factor | 0.285 |
| Rwork | 0.262 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ic8 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.837 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.071 | 0.508 |
| Number of reflections | 14184 | |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 98.5 | 93.9 |
| Redundancy | 9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | PEG 3000 7%, Li2S04 100 mM, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
