2NR7
Structural Genomics, the crystal structure of putative secretion activator protein from Porphyromonas gingivalis W83
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-13 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 94.537, 36.386, 62.474 |
Unit cell angles | 90.00, 119.75, 90.00 |
Refinement procedure
Resolution | 26.930 - 1.300 |
R-factor | 0.16419 |
Rwork | 0.162 |
R-free | 0.19665 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.324 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.930 | 1.310 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.064 | 0.410 |
Number of reflections | 42196 | |
<I/σ(I)> | 38 | 2.92 |
Completeness [%] | 92.5 | 68.4 |
Redundancy | 6.5 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 0.1M Bis Tris pH5.5, 25% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 277K |