2NQW
Structure of the transporter associated domain from PG_0272, a CBS domain protein from Porphyromonas gingivalis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-29 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97910, 0.97929 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.733, 45.195, 57.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.170 - 1.300 |
R-factor | 0.13157 |
Rwork | 0.130 |
R-free | 0.16715 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.601 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.200 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 20972 | |
<I/σ(I)> | 11.6 | 3.6 |
Completeness [%] | 94.6 | 69 |
Redundancy | 7.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 291 | 0.1M citric acid, 25% PEG 3350, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |