2NQC
Crystal structure of ig-like domain 23 from human filamin C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 52.141, 52.141, 69.216 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.170 - 2.050 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.288 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.170 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.070 | 0.298 |
| Number of reflections | 7027 | |
| <I/σ(I)> | 7.1 | 2.1 |
| Completeness [%] | 97.1 | 97.7 |
| Redundancy | 7.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 17.5% PEG 10000, 0.2M imidazole malate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
