2NQB
Drosophila Nucleosome Structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 93 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.143, 109.581, 182.045 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 99.000 - 2.300 |
Rwork | 0.223 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aoi |
RMSD bond length | 0.006 |
RMSD bond angle | 0.956 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.045 | 0.302 |
Number of reflections | 93949 | |
<I/σ(I)> | 22 | 3 |
Completeness [%] | 98.2 | 85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | Constituents of the crystallization buffer: Potassium Chloride, Manganese Chloride, and Potassium Cacodylate., pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | Potassium Chloride | ||
2 | 1 | 1 | Manganese Chloride | ||
3 | 1 | 1 | Potassium Cacodylate | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | Potassium Chloride | ||
6 | 1 | 2 | Manganese Chloride | ||
7 | 1 | 2 | Potassium Cacodylate | ||
8 | 1 | 2 | H2O |