2NPS
Crystal Structure of the Early Endosomal SNARE Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 252.882, 28.657, 41.887 |
| Unit cell angles | 90.00, 98.25, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.251 |
| Rwork | 0.247 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sfc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.277 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.124 | 0.490 |
| Number of reflections | 9365 | |
| <I/σ(I)> | 8.7 | 1.9 |
| Completeness [%] | 87.2 | 46.4 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1M trisodium citrate dihydrate, 36% (v/v) 2-methyl-2,4-pentandiol, 0.2M Li2SO4, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
