2NPF
Structure of eEF2 in complex with moriniafungin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.089 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.970, 159.120, 112.140 |
| Unit cell angles | 90.00, 97.83, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.900 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n0u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 45908 | |
| <I/σ(I)> | 17.5 | 4.9 |
| Completeness [%] | 99.5 | 98.2 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG, ethyleneglycol, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
