2NNY
Crystal structure of the Ets1 dimer DNA complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9756 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.556, 100.838, 69.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.580 |
| Rwork | 0.235 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k79 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.960 |
| High resolution limit [Å] | 2.580 |
| Rmerge | 0.080 |
| Number of reflections | 20507 |
| <I/σ(I)> | 20.45 |
| Completeness [%] | 95.8 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.2 M ammonium acetate 28% PEG 2000 0.1 M sodium citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 100K, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium acetate | ||
| 2 | 1 | 1 | PEG 2000 | ||
| 3 | 1 | 1 | sodium citrate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | ammonium acetate | ||
| 6 | 1 | 2 | PEG 2000 | ||
| 7 | 1 | 2 | sodium citrate | ||
| 8 | 1 | 2 | H2O |
