2NNW

Alternative conformations of Nop56/58-fibrillarin complex and implication for induced-fit assenly of box C/D RNPs

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Spacegroup name	P 31 1 2
Unit cell lengths	120.712, 120.712, 297.741
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.330 - 2.700
Rwork	0.234
R-free	0.26520
Structure solution method	MAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.330
High resolution limit [Å]	2.700
Number of reflections	68534

Crystallization Conditions

crystal ID	method	pH	temperature	details
1