2NNU
Crystal Structure of the Papillomavirus DNA Tethering Complex E2:Brd4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2005-10-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1159 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.892, 94.589, 119.691 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.590 |
| R-factor | 0.20133 |
| Rwork | 0.200 |
| R-free | 0.23331 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dto |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.427 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Number of reflections | 40019 | |
| <I/σ(I)> | 18.6 | 5 |
| Completeness [%] | 98.4 | 92.1 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 100mM citrate, 1.8-2.0M Na-acetate, 10mM DTT, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
