2NN4
Crystal structure of Bacillus subtilis yqgQ, Pfam DUF910
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-14 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.850, 41.250, 55.180 |
| Unit cell angles | 90.00, 113.39, 90.00 |
Refinement procedure
| Resolution | 47.590 - 2.100 |
| R-factor | 0.247 |
| Rwork | 0.247 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 12344 | |
| <I/σ(I)> | 12.2 | 2.8 |
| Completeness [%] | 97.5 | 84.7 |
| Redundancy | 6.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 278 | 0.066M sodium di-hydrogen phophate, 3.385M di-potassium hydrogen phosphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
