2NN2
Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 72.143, 72.143, 116.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.310 - 2.100 |
| R-factor | 0.21647 |
| Rwork | 0.214 |
| R-free | 0.26749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1buo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.642 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.310 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 19243 | |
| <I/σ(I)> | 15.45 | 3.08 |
| Completeness [%] | 96.7 | 95.6 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 0.7 M sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
