2NMS
The Crystal Structure of the Extracellular Domain of the Inhibitor Receptor Expressed on Myeloid Cells IREM-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-09-09 |
Detector | MAR CCD 225 mm |
Wavelength(s) | 0.873 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.230, 54.230, 72.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.670 - 2.600 |
Rwork | 0.218 |
R-free | 0.25500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zox |
RMSD bond length | 0.008 |
RMSD bond angle | 1.700 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.670 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.097 | 0.325 |
Number of reflections | 3936 | |
<I/σ(I)> | 0.0967 | 4.17 |
Completeness [%] | 97.8 | 95.7 |
Redundancy | 4.93 | 5.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.25M potassium thiocyanate, 25% (w/v) PEG MME 2000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |