2NMP
Crystal structure of human Cystathionine gamma lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.780, 107.570, 153.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 2.600 |
| R-factor | 0.18035 |
| Rwork | 0.177 |
| R-free | 0.24454 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n8p |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.640 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.140 | 0.338 |
| Number of reflections | 54383 | |
| <I/σ(I)> | 7.2 | 3.4 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 15% PEG3350, 200mM Ammonium Citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
