2NAD
HIGH RESOLUTION STRUCTURES OF HOLO AND APO FORMATE DEHYDROGENASE
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 115.970, 113.290, 63.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.050 |
| R-factor | 0.114 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.045 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.000 * | |
| Rmerge | 0.045 * | 0.075 * |
| Total number of observations | 206376 * | |
| Number of reflections | 51142 * | |
| Completeness [%] | 90.3 * | 84.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 50 (%) | precipitant |
| 2 | 1 | reservoir | MPD | 5 (%) | |
| 3 | 1 | reservoir | NAD | 5 (mM) | |
| 4 | 1 | reservoir | sodium azide | 5 (mM) |
