2MUC
MUCONATE CYCLOISOMERASE VARIANT F329I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | I 4 |
Unit cell lengths | 139.100, 139.100, 84.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.300 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.21600 |
Structure solution method | OTHER |
RMSD bond length | 0.009 |
RMSD bond angle | 24.100 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.300 |
Rmerge | 0.097 |
Number of reflections | 120580 |
Completeness [%] | 79.1 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | double dialysis * | 6.5 | pH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | MES | 20 (mM) | |
2 | 1 | 1 | 2 (mM) | ||
3 | 1 | 1 | 50 (mM) | ||
4 | 1 | 1 | 0.02 (%(w/v)) | ||
5 | 1 | 1 | beta-mercaptoethanol | 7 (mM) | |
6 | 1 | 1 | protein | 8 (mg/ml) |