2MSB

STRUCTURE OF A C-TYPE MANNOSE-BINDING PROTEIN COMPLEXED WITH AN OLIGOSACCHARIDE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	34.810, 85.160, 39.550
Unit cell angles	90.00, 97.41, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.174
Rwork	0.174
RMSD bond length	0.009
RMSD bond angle	1.400
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.700 *
Rmerge	0.070 *
Total number of observations	17511 *
Number of reflections	22750 *
Completeness [%]	91.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		10 (mM)
3	1	drop		5 (mM)
4	1	drop		1.1 (mM)
5	1	reservoir	PEG3350	15-20 (%)	or PEG8000
6	1	reservoir		20 (mM)
7	1	reservoir	Tris-HCl	100 (mM)
8	1	reservoir		10 (mM)
9	1	reservoir		0.02 (%)