2MLL

MISTLETOE LECTIN I FROM VISCUM ALBUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	107.648, 107.648, 311.278
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.248 *
Rwork	0.251
R-free	0.31900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aai
RMSD bond length	0.016
RMSD bond angle	0.066
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	10.000
High resolution limit [Å]	2.700	2.700
Rmerge	0.048 *	0.181 *
Total number of observations	334925 *
Number of reflections	29232
Completeness [%]	99.1	97.9 *
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5		pH 4.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	ammonium sulfate	40-45 (%)
3	1	reservoir		0.18 (M)
4	1	reservoir	acetate	0.1 (M)	or 0.1M phosphate