2JLZ

Dengue virus 4 NS3 helicase in complex with ssRNA and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Spacegroup name	C 1 2 1
Unit cell lengths	133.023, 104.794, 72.974
Unit cell angles	90.00, 115.91, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.19437
Rwork	0.192
R-free	0.23841
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jlu
RMSD bond length	0.008
RMSD bond angle	1.237
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.900	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.580
Number of reflections	193997
<I/σ(I)>	13.3	2.3
Completeness [%]	100.0	100
Redundancy	4.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1