2JLP
Crystal structure of human extracellular copper-zinc superoxide dismutase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.738, 93.588, 75.600 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 28.200 - 1.700 |
| R-factor | 0.152 |
| Rwork | 0.150 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9v |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 81056 | |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 97.3 | 80 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PEG3350, THIOCYANATE, BIS-TRIS PROPANE PH 7.5 |
