2JL9

Structural explanation for the role of Mn in the activity of phi6 RNA- dependent RNA polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	106.519, 91.599, 142.705
Unit cell angles	90.00, 101.64, 90.00

Refinement procedure

Resolution	19.915 - 3.200
R-factor	0.2177
Rwork	0.215
R-free	0.27200
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.280
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.300
High resolution limit [Å]	3.200	3.200
Rmerge	0.180
Number of reflections	40323
<I/σ(I)>	7
Completeness [%]	91.0	81
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1