2JKX
Galactose oxidase. MatGO. Copper free, expressed in Pichia Pastoris.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.950, 88.520, 85.350 |
| Unit cell angles | 90.00, 117.24, 90.00 |
Refinement procedure
| Resolution | 38.240 - 1.840 |
| R-factor | 0.167 |
| Rwork | 0.163 |
| R-free | 0.20100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gog |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.265 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.300 | 1.900 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.040 | 0.150 |
| Number of reflections | 53734 | |
| <I/σ(I)> | 12.8 | 4.1 |
| Completeness [%] | 96.6 | 85.5 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.9 | 18% PEF 8000, 100MM MES PH5.9, 200MM CALCIUM ACETATE |
