2JKI

Complex of Hsp90 N-terminal and Sgt1 CS domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-03-19
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	100.268, 129.654, 135.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.072 - 3.300
R-factor	0.2009
Rwork	0.199
R-free	0.23830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1AMW 1rl1
RMSD bond length	0.010
RMSD bond angle	1.480
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.170	0.720
Number of reflections	29660
<I/σ(I)>	7.9	1.7
Completeness [%]	99.3	98
Redundancy	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	277	INITIAL MULTIPLE CRYSTALS WERE GROWN BY VAPOR DIFFUSION AT 4 C AGAINST 26% W/V PEG4000, 100 MM TRIS (PH 8.5), AND 200 MM MAGNESIUM SULPHATE. SUBSEQUENT STREAK SEEDING INTO SOLUTIONS OF 16% W/V PEG4000, 100 MM TRIS (PH 8.5), AND 200 MM MAGNESIUM SULFATE PRODUCED SINGLE THIN PLATES.