2JK9
The structure of splA-ryanodine receptor domain and SOCS box containing 1 in complex with a PAR-4 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-29 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.523, 82.957, 38.540 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 83.050 - 1.790 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A HOMOLOGY MODEL FROM PDB ENTRY 2FNJ |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.321 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.320 | 1.810 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.070 | 0.540 |
| Number of reflections | 20678 | |
| <I/σ(I)> | 12.64 | 1.67 |
| Completeness [%] | 96.2 | 91.4 |
| Redundancy | 3.48 | 3.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% PEG3350, 0.2M NACL, 0.1M BIS-TRIS PH 5.5 |
