2JJU
Structure of human signal regulatory protein (sirp) beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.166, 65.172, 58.113 |
| Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
| Resolution | 32.110 - 1.190 |
| R-factor | 0.14 |
| Rwork | 0.139 |
| R-free | 0.15700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uv3 CHAIN A |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.263 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 76112 | |
| <I/σ(I)> | 36.3 | 3.3 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 8.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 400 NL 25 MG/ML SIRP BETA PLUS 50 NL RESERVOIR (2.5 M AMMONIUM SULPHATE, 0.1 M BIS-TRIS PROPANE PH 7.0) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 20.5 C. |
