2JJS

Structure of human CD47 in complex with human signal regulatory protein (SIRP) alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	42.477, 72.410, 89.826
Unit cell angles	90.00, 95.01, 90.00

Refinement procedure

Resolution	89.440 - 1.850
R-factor	0.171
Rwork	0.169
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2UV3 FOR CHAINS A AND B PDB ENTRY 2ICC FOR CHAINS C AND D
RMSD bond length	0.010
RMSD bond angle	1.269
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.800	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.140	0.790
Number of reflections	46373
<I/σ(I)>	17.6	2.8
Completeness [%]	100.0	100
Redundancy	10.3	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.4	278	100 NL SIRP ALPHA / CD47 COMPLEX (1:1 RATIO, EACH PROTEIN AT APPROX. 0.375 UM) PLUS 100 NL RESERVOIR (0.2 M POTASSIUM IODIDE, 20% W/V PEG 3350) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 5 C., pH 7.4
1		7.4	278	100 NL SIRP ALPHA / CD47 COMPLEX (1:1 RATIO, EACH PROTEIN AT APPROX. 0.375 UM) PLUS 100 NL RESERVOIR (0.2 M POTASSIUM IODIDE, 20% W/V PEG 3350) EQUILIBRATED AGAINST 95 UL OF RESERVOIR AT 5 C., pH 7.4