2JJD
Protein Tyrosine Phosphatase, Receptor Type, E isoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.020, 123.616, 219.116 |
| Unit cell angles | 90.00, 91.13, 90.00 |
Refinement procedure
| Resolution | 218.220 - 3.200 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.169 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.650 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.140 | 0.560 |
| Number of reflections | 373908 | |
| <I/σ(I)> | 9 | 1.5 |
| Completeness [%] | 98.7 | 92.4 |
| Redundancy | 3.4 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.60 M MGSO4, 0.1 M MES PH 6.5 |
