2JHU
CRYSTAL STRUCTURE OF RHOGDI E154A,E155A MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-02 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 130.775, 130.775, 162.315 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.850 - 1.650 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.070 | 0.800 |
| Number of reflections | 63939 | |
| <I/σ(I)> | 40.5 | 2 |
| Completeness [%] | 99.0 | 90.5 |
| Redundancy | 18.2 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.0 M AMMONIUM SULFATE 0.2 M SODIUM CHLORIDE 0.1 M SODIUM CACODYLATE PH 6.5 |
